Journal: Genome Biology

Article Title: Highly recurrent CBS epimutations in gastric cancer CpG island methylator phenotypes and inflammation

doi: 10.1186/s13059-021-02375-2

Figure Lengend Snippet: CBS epimutations associate with CIMP in primary GCs. a Overlap of genes that are promoter hypermethylated and downregulated at the RNA level in the discovery GC cell line panel, TCGA-stomach adenocarcinoma [STAD] and Singapore [SG] cohorts ( β -value difference ≥ 0.3 and q -value < 0.05) [left panel]. Average promoter methylation β -values and gene expression of CBS gene in STAD according to CIMP subtypes (* P < 0.001, two-tailed Wilcoxon rank sum test, each CIMP group vs. non-CIMP group) [right panel]. b Immunohistochemistry of CBS in a normal human stomach with black arrow indicating cytoplasmic staining in epithelial cells. Control sections were not treated with the primary antibody. c Summary of CBS staining in 66 cases of matched normal and gastric adenocarcinomas (* P < 0.05, two-tailed Fisher’s exact test) [left panel] and an example of a matched normal vs. tumor case with a negative score [right panel]. d Summary of somatic and germline genetic alterations at CBS in STAD. PALP, pyridoxal-phosphate dependent enzyme domain; CBS, cystathionine beta-synthase domain; aa, amino acid

Article Snippet: Normal human stomach antrum and fundus tissue slides were purchased from Novus Biologicals (NBP2-30203, NBP2-30204).

Techniques: Methylation, Gene Expression, Two Tailed Test, Immunohistochemistry, Staining, Control

Journal: Cancers

Article Title: DUOX2, a New Biomarker for Disseminated Gastric Cancer’s Response to Low Dose Radiation in Mice

doi: 10.3390/cancers13164186

Figure Lengend Snippet: TCGA data of DUOX2 mRNA expression in human Stomach adenocarcinoma (STAD) tumors. ( A ) Levels of DUOX2 mRNA expression in 34 Normal and 415 Primary stomach adenocarcinoma. ( B ) Levels of DUOX2 mRNA expression based on tumor grade. The number of samples of each tumor grade are indicated in parenthesis. ( C ) Levels of DUOX2 mRNA expression based on nodal metastasis status. ( D ) Expression of DUOX2 mRNA based on individual cancer stages. Data provided by the UALCAN web resource for analyzing cancer OMICS data ( http://ualcan.path.uab.edu/index.html , accessed on 28 July 2021). Significance of difference estimated by Student’s t -test considering unequal variance. * p < 0.05.

Article Snippet: Human stomach adenocarcinoma tissue arrays (37 samples) with progressive disease were obtained from Pantomics, Inc. (Cat no, STC 962, Richmond, CA, USA) and analyzed by immunohistochemistry for DUOX2 (mouse monoclonal MIL-MABN787, 1/300, Millipore Sigma, St-Louis, MO, USA), and macrophages (CD68, mouse monoclonal EBS-14-0688-82, 1/1000).

Techniques: Expressing

Journal: Cancers

Article Title: DUOX2, a New Biomarker for Disseminated Gastric Cancer’s Response to Low Dose Radiation in Mice

doi: 10.3390/cancers13164186

Figure Lengend Snippet: Immuno-Histo-Chemistry (IHC) in 37 human adenocarcinoma samples. ( A ) Staining scored blinded by a pathologist at Pantomics, Inc. Staining > 2.8 was considered strong DUOX2 positive, score of “0” was considered DUOX2 negative. Percentage of strong staining and negative DUOX2 staining are indicated. ( B ) Example of heterogeneous (positive and negative) DUOX2 staining in well differentiated/low grade gastric adenocarcinoma (Malignant 1). Red arrows indicate cells expressing DUOX2. ( C ) Expanded view of inset from ( B ). ( D ) Example of strong DUOX2 staining in poorly differentiated/high grade stomach adenocarcinoma. ( E ) Intensity of CD68 staining in DUOX2 negative and positive samples. Student’s t -test, *** p = 0.0005. ( F ) CD68, red arrows indicate macrophages tumor and stroma infiltration in same tissue as ( B ) expressing DUOX2. ( G ) Expanded view of inset from ( F ). ( H ) IHC in poorly differentiated/high grade stomach adenocarcinoma showing strong CD68 staining. Images dimensions are 450 × 383 pixels (W × H).

Article Snippet: Human stomach adenocarcinoma tissue arrays (37 samples) with progressive disease were obtained from Pantomics, Inc. (Cat no, STC 962, Richmond, CA, USA) and analyzed by immunohistochemistry for DUOX2 (mouse monoclonal MIL-MABN787, 1/300, Millipore Sigma, St-Louis, MO, USA), and macrophages (CD68, mouse monoclonal EBS-14-0688-82, 1/1000).

Techniques: Staining, Expressing

Journal: Cancers

Article Title: DUOX2, a New Biomarker for Disseminated Gastric Cancer’s Response to Low Dose Radiation in Mice

doi: 10.3390/cancers13164186

Figure Lengend Snippet: Stat-1 response to mDCF and LDFRT in NCI-N87 cells. ( A ) Western blot analysis of total Stat-1, and Stat-1 phosphorylated at Serine 727 (p-Ser727) and Tyrosine 701 (p-Tyr701). Cells were exposed to the indicated treatment and pre-treated (+) or not (−) with the Src kinase inhibitor Dasatinib. ( B – D ) Fold induction measured by densitometry and normalized to Actin and the untreated sample (Vehicle). ( E ) Expression of Stat1 in normal and stomach adenocarcinoma (STAD) from TCGA samples accessed on 28 July 2021. . Student’s t -test, *** p < 0.0005.

Article Snippet: Human stomach adenocarcinoma tissue arrays (37 samples) with progressive disease were obtained from Pantomics, Inc. (Cat no, STC 962, Richmond, CA, USA) and analyzed by immunohistochemistry for DUOX2 (mouse monoclonal MIL-MABN787, 1/300, Millipore Sigma, St-Louis, MO, USA), and macrophages (CD68, mouse monoclonal EBS-14-0688-82, 1/1000).

Techniques: Western Blot, Expressing